158 PATHOGENIC BACTERIA. 
X. ACTINOMYCOSIS. 
1. Study the morphology and biology of cultures 
of Actinomyces bovis. 
2. Prepare sections from tissue containing colo- 
nies of the parasite. 
3. Stain by Gram’s method or as follows : 
Fic. 90.—Actinomyces (von Jaksch). 
(a) Stain deeply in saturated aqueous eosin solu- 
tion for ten minutes. 
(4) Wash in water. 
(c) Stain in anilin-gentian-violet for two to five 
minutes. 
(2) Wash in normal salt solution.’ 
(e) Treat with iodin solution (iodine, 1 part; 
potassium iodid, 2 parts ; distilled water, 100 parts), 
for one minute. 
(f) Wash in water and dry slightly with filter- 
paper. 
1 See Appendix, page 173. 
