MALARIA. 159 
(g) Clear in anilin oil, then in several changes 
of xylol. 
(2) Mount in balsam. 
XI. MALARIA. 
ie 
I. Examination of Fresh Blood for the Ma- 
larial Hematozoon. 
(a) Clean a slide and cover-glass. 
(4) Place a small drop of the blood to be ex- 
amined on the slide, cover, and seal with vaselin, 
(c) Examine with the oil-immersion lens. 
The ameboid movements of the Hematozoon 
malariz are visible at the room-temperature, but 
become more active if the slide is warmed. 
2. Stained Preparations. 
(1) First Method. 
(a) Place a small drop of blood between two 
cover-glasses, slide the covers apart, and allow the 
films to dry. 
(4) Fix by placing in absolute alcohol for five 
minutes or in the vapor of formaldehyd for two 
and a half minutes, or by one of the methods given 
under Anthrax (page 131). 
(c) Stain in a I per cent. aqueous solution of eosin 
for five minutes or, if formaldehyd is used for fixa- 
tion, in an alcoholic (60-75 per cent.) solution of 
eosin for three to ten minutes. 
(2) Contrast-stain in saturated aqueous solution 
of methylene-blue for five minutes for the alcoholic 
preparations, for one minute for the formaldehyd 
