-STAINS AND REAGENTS. 171 
The watery solutions soon decompose, and must 
be made only as required for use. Methylene-blue, 
however, may be made up as a saturated aqueous 
solution, as it is permanent. Always filter a stain 
before use. 
2. Kuhne’s Methylene-blue. 
Methylene-blue, 1.5 gm.; 
Absolute alcohol, IO CC} 
Carbolic acid (x : 20), 100 ¢.c. 
Stain films five minutes. 
3. Loffler’s Methylene-blue. 
Saturated alcoholic solution of 
methylene-blue, 20 G8} 
Solution of potassium hydrate 
in water (1: 10,000), 100 ¢.¢. 
- 4, Carbol-thionin-blue. 
Thionin-blue, I gm.; 
Carbolic acid (1 : 40), 100 ¢.c. 
Dilute 1 volume of the stain with 3 of water, 
when required for use. Stain from three to 
five minutes. 
5. Anilin-water Solution. 
Anilin oil, 5 .¢.c.5 
Water, IOO ¢c.c. 
Shake together, allow to stand for five minutes, 
and filter through a moistened filter. 
6. Anilin-gentian-violet and Anilin-fuchsin. 
Anilin-water, Io parts; 
Saturated alcoholic solution of 
gentian-violet or fuchsin, I part. 
