172 
APPENDIX. 
7. Gram’s Iodin Solution. 
Todin, I part; 
Potassium iodid, 2 parts; 
Distilled water, 300° 
8. Ziehl’s Carbol-fuchsin. 
Basic fuchsin, I part; 
Absolute alcohol, 10 parts; 
Carbolic acid (1 : 20), Ioo ‘ 
9g. Gabbet’s Blue. 
Sulphuric acid (25 per cent. 
solution), 100 ¢.¢.} 
Methylene-blue, 2 emi. 
Allow the diluted acid to stand twenty-four 
hours or until it is cold before adding the 
methylene-blue. 
ro. Unna’s Polychrome Methylene-blue. 
Methylene-blue, I part ; 
Potassium carbonate, Go? 
Water, 100 parts. 
Must be ripened for months. The ripéned 
solution may be procured from Griibler. 
1. Ehrlich’s Triacid Stain. 
Saturated aqueous solution 
of orange G, 120 parts. 
Acid fuchsin, 80“ 
Methyl-green, too (‘ 
Distilled water, 300. «‘S 
Absolute alcohol, 180‘ 
Glycerin, 50 ‘ 
