STAINS AND REAGENTS. 173 
Never shake the solution. Pipet from the top 
what is needed for use. 
12. Ehrlich-Biondi Stain. 
Is best procured ready made from Griibler: 
13. Chenzinsky-Plein Stain. 
Saturated aqueous solution of 
methylene-blue, 40 CC. 5 
Alcoholic (70 per cent.) solu- 
tion of eosin (1 : 200), 2G cue: 2 
Distilled -water, 40 c.c. 
The best results are obtained by staining’ for 
twenty-four hours. Fairly good results may 
be obtained by using warmed stains for 
fifteen minutes. 
14. Normal Salt Solution. 
Distilled water, 100 ¢.C.; 
Sodium chlorid, 0.75 gm. 
15. Acid Alcohol. 
Alcohol (7o per cent.), 97 C.C. 5 
Hydrochloric acid, oe 
16. Zenker’s Fluid. 
Potassium bichromate, 2.5 gm.; 
Sodium sulphate, I gm.; 
Corrosive sublimate, 5 gm.; 
Glacial acetic acid, 5c.c.3 
Water, ad 100 ¢.c¢. 
Do not add the acetic acid until ready for use. 
Fix tissues from one to twenty-four hours. 
