[Vol. 1 



394 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



infrequent on the Lancashire coast, although many forms ap- 

 proach it very closely and differ only in this one feature. As 

 will be seen below, certain other plants agreed with de Vries's 

 Lamarckiana except in the shape of the buds. 



In 1911 a sowing of the seeds yielded 22 plants. The rosettes 

 were for the most part uniform and very similar to (E. La- 

 marckiana, two, however, having red midribs and lighter green 

 leaves (rubrinervis type). One plant was aberrant, resembling 

 CE. mut. semilata in its buds, which were, however, small as in 

 (E. biennis. The bud cone was also somewhat rounded and 

 barrel-shaped, length of ovary 11 mm., hypanthium 37 mm., 

 cone 19 mm., petals 22 mm., style short so that anthers sur- 

 round base of stigma. The features of this plant make it 

 scarcely likely that it arose as a hybrid. It produced plenty of 

 poUen and seeds. 



Another sowing of these seeds in 1912 yielded 140 plants, 

 which included one mut. lata with bad pollen (doubtless having 

 15 chromosomes) and one variegated Lamarckiana plant. The 

 variegation was noticed when the plant was a young seedling. 

 It reached maturity and proved to be a periclinal chimsera. 

 Nearly all the leaves were variegated green and yellow. Many 

 leaves were green bordered with yellow, showing the absence of 

 chloroplasts from the epidermal and probably also the hypo- 

 dermal layer. Occasional leaves were almost entirely yellow, 

 and some were yellow on one side of the midrib and green on 

 the other. There were also broad white bands on the margin 

 of the sepals. The pollen was abundant and plenty of seeds 

 were set. 



Two sowings of seeds from this plant were made in 1912. 

 The seeds numbered respectively 121 and 145. Only two seeds 

 in one pan were observed to germinate, and the seedlings quickly 

 died, probably from lack of chlorophyll. Regarding the origin 

 of this periclinal mutation, it would appear to have originated 

 in the embryo after fertilization through the loss of chloroplasts 

 from the outer layers of the growing point. 



The foliage in the rest of the culture agreed with the type of 

 (E. Lamarckiana. One plant differed in having stem-leaves more 

 or less pointed at the base, not crinkled, midribs pink, and smaller 

 flowers (petals 29 mm. long x 38 mm. broad, style short, buds 



