164 



Tannic Acid Fermentation 



TABLE A. 



ORGANISM 



10. 



11. 



1 . Penicillium brevicaule .... 



2. Penicillium camemherti. . . 



3. Penicillium clavi forme.. . . 



4. Penicillium duclauxii 



5. Penicillium granulatum. . . 



6. Penicillium italicum 



7. Penicillium lilacinum . 



8. Penicillium purpurogenum 



9. Penicillium sp 



Aspergillus flavus 



Aspergillus niger 



12. Trichoderma lignorum . 



13. Mucor circinelloides . . . 



14. Mucor rouxii 



15. Mucor spinosus 



16.. Polyporus sulphureus . . 



17. Polyporus resinosus. . . 



18. Fames megaloma 



19. Chaetomium sp 



20. Chaetostylon sp 



21. Stysanus sp 



22. Cephalothecium roseum 



23. Circinella umhellata. . . 



CHARACTEB OF GROWTH 



0.25 per cent 2 per cent 5 per cent 10 per cent 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



good 



no growth 

 good growth 

 good growth 

 good growth 



good 



good 



good 



good 



good 



good 



slight 



slight 



none 



good 



good 



good 



none 



slight 



slight 



none 



none 



none 



none 



none 



none 



none 



none 



good 



good 



very slight 



good 



none 



good 



slight 



none 



none 



good 



good 



good 



none 



none 



none 



none 



none 



none 



none 



none 



none 



none 



none 



none 



good 

 good 

 good 



Erlenmeyer flasks of 150 cc. capacity were employed, and in each 

 were placed 50 cc. of the culture solution. After . sterilization 

 these flasks were inoculated and maintained at room temperature. 

 In addition to the above list of organisms the following were tested: 

 Aspergillus oryzae, Nectria ipomoeae, Fusarium oxysporum, Phyco- 

 myces nitens and Stilbella sp. Of all organisms tested only Asper- 

 gillus niger and Penicillium sp.^<> developed. Duplicate cultures 

 of all these organisms on Chinese galls gave similar results, except 

 in this case Aspergillus flavus produced a very slight growth. 



VanTieghem" found that both Aspergillus niger and Penicillium 

 glaucum could withstand a saturated solution of tannic acid, and 



2« One other species of Penicillium, as indicated in the appendix, is able 

 to develop upon a 10 per cent tannic acid solution. 

 2' Loc. cit. 



