SUPPURATION. 87 



caused the formation of pus, and the preparations from the liver were 

 found to be specially potent. Similar preparations from blood and 

 egg yolk were active, while those from fibrin and the white of egg 

 had no effect. Hemi-albumose was also found to be active, and this 

 fact is placed in contrast with the negative result obtained with 

 pepton. 



The bacterial proteids, also some of the vegetable proteids, when 

 injected directly into the blood, cause a general leucocytosis. A 

 very small amount of the proteid of the pyocyaneus injected under 

 the skin of the forearm caused the following symptoms : Two hours 

 after the injection there was marked pain along the lymphatics, es- 

 pecially localized in the elbow and axilla. The temperature showed 

 no marked elevation. On the following day there was observed a 

 distinct erysipelatous redness and swelling extending for some 

 inches about the place of injection, and this was accompanied by 

 severe pain. The inflamed area felt hard, and projected distinctly 

 above the surrounding surface, and the lymphatics of the arm ap- 

 peared like red cords. On the third day the swelling and redness 

 were more marked, and extended from the wrist to the elbow ; but 

 on the fourth day the symptoms began to recede. Here we have 

 clinically a typical erysipelas with lymphangitis, and Buchner 

 claims that all the cardinal symptoms of inflammation — rubor, calor, 

 dolor, tumor — could not be produced without involvement of the 

 solid tissues. Similar, but less marked, symptoms were induced by 

 the injection of a dilute solution of vegetable casein. 



Buchner states that bacteria can not cause inflammation unless 

 they be broken down. The pyogenetic substance contained within 

 the bacterial cell can have no chemotactic action until the cell disin- 

 tegrates. Thus, the anthrax bacillus contains a pyogenetic substance, 

 but no pus is formed in mice with anthrax, because there is no de- 

 struction of the bacilli. The pyogenetic proteid of the anthrax 

 bacillus, however, manifests its action in malignant pustule. 



Many non-pathogenic germs may grow in wounds and by elabor- 

 ating their poisons may increase and influence the general intoxica- 

 tion. Brunner has found the proteus vulgaris growing in a wound, 

 and it is well known that the products of this germ are powerful 

 poisons. 



Mannotti, afl«r treating animals with sterilized pus, states the 

 following conclusions : 



(1) Sterilized pus has substantially the same toxic properties as 

 sterilized cultures of the staphylococcus. (2) Kepeated injections of 

 sterilized pus induce chronic intoxication and marasmus. (3) Injec- 

 tion under the skin causes a specially grave form of poisoning. (4) 

 The symptoms and pathological lesions caused by these injections 

 correspond with those observed in men suffering from chronic sup- 

 puration. 



