THE LYSmS. 135 



part of the defibrinated blood of the rabbit was added to two or 

 three parts of the active serum, the mixture became, within two or 

 three minutes, red, clear and limpid. Microscopical exammation of 

 this fluid showed only the stroma of the globules, more or less de- 

 formed, transparent, and scarcely visible. (3) When the active 

 serum of the guinea-pig was heated to 56° for half an hour (or for 

 less time at 60°) it lost its property of dissolving the corpuscles of 

 the rabbit but still agglutinated them. (4) If to a mixture of the 

 defibrinated blood of the rabbit and the serum of the guinea-pig, 

 the latter having been heated to 55°, there was added a certain 

 quantity of fresh serum from either a normal guinea-pig or rabbit 

 the hemolytic property of the serum was restored. (5) It follows 

 from the above that the destruction of the hemolytic properties of 

 the serum of the guinea-pig is only partial and the injury done this 

 serum by heat is partially at least repaired by the addition of fresh 

 serum from either a normal rabbit or a normal guinea-pig. (6) The 

 serum of an untreated guinea-pig has only feeble agglutinating 

 power on the corpuscles of the rat and has no solvent action on 

 these bodies. (7) The active serum of the treated guinea-pig was 

 without influence on the defibrinated blood of another guinea-pig, 

 also without action on the corpuscles of the pigeon. It agglu^- 

 tinated markedly the corpuscles of the rat and the mouse. (8) 

 When two c.c. of the defibrinated blood of the rabbit were intro- 

 duced into the peritoneal cavity of a guinea-pig which had been 

 immunized to th e blood of the rabbit, the corpuscles thus introduced 

 were quickly and completely destroyed. The liquid removed from 

 the cavity after about ten minutes was found to be uniformly red 

 and perfectly clear and limpid. (9) If the blood of the rabbit 

 mixed with a small quantity of the serum of the treated guinea- 

 pig which had been heated to 55° was injected into the peritoneal 

 cavity of the treated guinea-pig, the phenomenon of destruction 

 took place very rapidly. (10) The serum of the guinea-pig injected 

 intravenously into the rabbit proved to be highly toxic. 



Bordet ^ has made additional valuable contributions to the subject 

 of hemolysis. The facts ascertained in these researches agree prac- 

 tically in all details with those already given concerning the work of 

 Ehrlich and Morgenroth, but Bordet's explanation of the facts 

 differs from that given by the German investigators. Ehrlich's 

 intermediary body is designated by Bordet as the sensitizer (substance 

 sensihitairice), and its function is to render impressionable globules 

 sensitive to the action of the toxic body, which Ehrlich designates as 

 complement and Bordet calls alexin. Ehrlich holds that the combi- 

 nation between the corpuscles and the intermediary body is a chem- 

 ical one, while Bordet explains the action of his sensitizer on phys- 

 ical grounds. The term alexin, adopted by Bordet, is the same as 

 ■ Annales de PlnstitiU Pasteur, 1899 and 1900. 



