210 MISCELLANEOUS EXAMPLES 



is still densely packed with the fission products of Chlorochytrium 

 — and such transformed elements may be seen presenting them- 

 selves either in the centre of the mass or at its periphery. The 

 typical colours of the Anabena show themselves first in separate 

 corpuscles, while these corpuscles subsequently multiply so as 

 to produce the necklace-like chains. 



In Fig. 31 ( X 37S) some illustrations will be found of the foregoing 

 statements ; thus A shows a space in which some small, and several 

 large, green Chlorochytrium segments were found in association 

 with purple Anabena corpuscles ; in B a larger space is shown, in 

 which there was a nearly equal and diffused admixture of the green 

 Chlorochytrium together with purple and blue Anabena elements ; 

 in C a space is represented, distended by Anabena chains, which 

 showed under the microscope only three or four minute Chloro- 

 chytrium segments remaining ; while in D some of the blue 

 Anabena is shown as it was growing free, after having burst 

 through some of the cells at the broken edge of one of the 

 leaflets of the Lemna. 



Seeing that the spores of Anabena are non-motile they would 

 have no means of getting into these closed cells, and, apart from 

 this general consideration, there is the fact that the change of 

 colour and heterogenetic transformation has been actually seen 

 occurring here and there in the midst of tightly-packed 

 Chlorochytrium cells. 



(c) On the Origin of Anabena from the Chlorophyll 

 Corpuscles of Lemna trisulca. 



An isolated, decolourised leaflet of Lemna trisulca was found in 

 which, along the centre and towards its proximal extremity, green 

 chlorophyll corpuscles were still to be seen lining the spherical, 

 sub-epidermal cells. In two separate, lateral regions of this leaflet 

 ill-defined patches were found in which the chlorophyll corpuscles 

 were mostly decolourised, and showed only a few colourless 

 granules in their interior. Intermixed among them, however, 

 there were other chlorophyll corpuscles which still preserved 

 their green colour. A portion of one of these patches is shown 

 under a low power in Fig. 32, A (x 250). 



As I wished to see what further changes these corpuscles would 

 undergo, I placed this leaflet alone in a small tube with some fresh 

 water, and left it under a bell-glass on my mantel-piece. Three 



