4l6 APPENDIX. 



I). Colonies confluent, growing luxuriantly. 

 (a) Cocci arranged irregularly. 



(/3) Cocci occur as diplococci, or dumb bell- shaped organisms, 

 (y) Cocci arranged as sarcinas. 



a. Cocci arranged irregularly. 



(1) Micrococcus candicans forms irregular masses, small yellowish white 

 discs with smooth margins in substance of gelatine ; opalescent or milk 

 white moist flat colonies 2mm. or more in diameter, with indented and 

 sinuous margins at the surface ; dark brown in the centre when seen by 

 transmitted light, but transparent near the thin margin ; nail-head appear- 

 ance in puncture cultivations ; micrococci quite round, moderately large. 



(2) Micrococcus urece. — Grows as miliary points like mother-of-pearl, 

 smooth on surface, sharp margins; these grow rapidly, are well formed 

 in twenty-four hours ; project above surface of gelatine, colony gradually 

 divided by fissures ; along the track of the needle in a tube culture there 

 appear long delicate threads ; there is a large surface growth ; has a peculiar 

 paste-like odour ; grows best at higher temperatures, (30° C), coccus 0.8 to 

 ifi in diameter, occurs as diplococci, tetrads, or chains ; along with other 

 organisms, causes decomposition of urea into ammonium carbonate. 



, (3) Staphylococcus cereus albus grow moderately rapidly ; white points on 

 gelatine during the first few days ; in stroke cultivations forms a wax-like 

 layer, with slightly thickened irregular margins along the needle track ; 

 grows on blood serum and potato ; found in pus ; usually saprophytic 

 in its action. 



/3. Cocci arranged as diplococci, or dumb-bell shaped organisms. 



(1) Diplococcus lacteus famformis grows rapidly along track of needle 

 in small points, which run together to form milk white colonies ; found in 

 the sputum and certain secretions as isolated diplococci ; in cultivations 

 occurs as parallel bands of diplococci, each organism being about 1.25/i in 

 length and consisting of two hemispheres, between which there is a distinct 

 but narrow fissure. 



(2) Diplococcus albicans amplus. — Very like No. I, found in the same 

 positions, but grows in thick white lines along the track of a stroke inocula- 

 tion, organism is comparatively large, measuring 2.2S/U in diameter. 



(3) Diplococcus der Pferdepneumonie. — Obtained from the lungs of a horse 

 affected with acute pneumonia. Has only been cultivated on gelatine and 

 agar-agar at the temperature of the room ; forms small white, somewhat 

 transparent rounded colonies in agar-agar ; along the line of the needle 

 track in a gelatine tube culture there is seen a row of small white granules, 

 which gradually become larger and coalesce, but there is no special surface 

 growth ; this is an oval micrococcus which sub-divides in its shortest 

 diameter, two of them usually lying together surrounded by a clear homo- 

 geneous capsule ; pathogenic for mice, guinea-pigs, rabbits, and dogs. 



y. Cocci arranged as sarcinse. 

 (l) Micrococcus tetragomts forms small white points in gelatine in from 

 twenty-four to twenty-eight hours ; under lens deep colonies have a faint 

 yellow tinge ; mulberry-like surface ; is somewhat raised on the surface of 

 the gelatine along track of needle in puncture inoculation ; first there appear 

 rounded points, which run together ; these grow most readily at surface. 



