MOUNTING AND PREPARATION OF OBJECTS. 5" 



in water till the stain is removed from the cytoplasm 

 and passed through 30%, 50%, and 70% alcohol. At 

 this stage it is immersed for a few seconds in a solution 

 of eosin in 70% alcohol to stain the cytoplasm, and is 

 then passed rapidly up through 90% and 95% alcohol 

 and xylol to be mounted in balsam. The nuclei should 

 be dark blue and the cytoplasm reddish yellow. 



Another good stain for the beginner to use, though not 

 to make up, is the Wright modification of Leishman's 

 blood-stain, which with a single solution yields a most 

 beautiful differentiation of half a dozen cell elements. 

 Its use is described more fully in Chapter IX. 



REFERENCES. 



Chamberlain, C. J. Methods in Plant Histology. Chicago, 1901. 

 Gage, S. H. The Microscope. Ithaca, N. Y., 1904. 

 Lee, a. B. The Microtomists' Vade Mecum. Philadelphia, 1900. 

 ZiMMEEMANN, A. Botanical Microtechnique. Humphrey, J. E., 

 trans. New York, 1893. 



