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REPORT ON THE DISEASES OP SILKWORMS IN INDIA 



APPENDIX IV. 



Bacillus A. 



Morphology . . Stout rod 3 — 6[i long x 0-75 — 0'9(i wide. Rounded ends. 



Motility . . Rather slowly moving. 



Spore formation . Abundant — central. 



Gram . . . Positive. 



Agar plate . . Dirty white, rather irregular, curled, at first moist becoming rather 



dry, very membranous. 



Agar slope . . Dirty white, becoming wrinkled. 



Gelatine stab. . Liquified, saccate, pellicle on surface. 



Potato . . . Greyish, elevated, dull. 



Nutrient broth . Turbid with slight ring like pellide. 



Litmus milk . . No change at first — slight coagulation after 1 week. 



Dextrose . . No gas. 



Lactose . . No gas. 



Habitat . . Diseased muga and mulberry caterpillars ; healthy muga, eri and 



mulberry caterpillars ; mulberry and castor leaf. . 



, Probably Bacillus megaterium bombycis. 



Bacillus B. 



Morphology . . Large rod 3 — 6[a longx0-75[* wide, more slender than A. 



Motility . . Moderately to actively motile. 



Spore formation . Abundant, central. 



•Gram . . . Positive. 



Agar plate . . Whitish, irregular to roundish, curled, very membranous. 



Agar slope . . Pearly white, rather spreading, with root like outgrowths, wrink- 



led. 



•Gelatine stab . Root-like outgrowths from line of inoculation, then liquifying. 



Potato . . . Greyish, dull, raised, not very abundant at first. 



Nutrient broth . Pellicle round edge later becoming dense. 



Litmus milk . . A little acid after 3 days. 



Dextrose . . No gas. 



Lactose . . No gas. 



Habitat . . Dead moths. 



Probably Bacillus ramosus (mycoides). 



Bacillus C. 



Morphology . . Very short rods 1'5U longx0 - 6n wide. 



Motility . . Actively motile. 



Spore formation . None. 



Gram . . . Negative. 



Agar plate . . Roundish, white. 



Agar slope . . Moist, shining, white, filiform. 



Gelatine stab . Not liquified, filiform. 



Potato . . . Elevated, yellowish. 



Nutrient broth . Turbid. 



Litmus milk . . Coagulated, acid. 



