88 BACTERIOLOGICAL, EXAMINATION OF MIL,K. 



For the preparation of the Eisner culture medi 500 

 grams of potatoes are extracted with a liter of water ; to 

 this extract 100 grams of gelatine are added ; 10 c. c. of 

 the potato gelatine is made alkaline with 2.5 — 3 c. c. of 

 i-io normal sodium hydroxide mixed with i per cent po- 

 tassium iodide. 



The methods of cultivation of the known species, as 

 well as the possible occurring pathogenic staphylococcus, 

 and streptococcus, the streptococcus of infectious indura- 

 tion or the Streptococcus agalactiae contagiosae — the 

 cause of chronic and acute mastitis of the cow — the 

 Staphylococcus and Streptococcus Guillebeau, the bacil- 

 lus Guillebeau, etc., need no special explanation ; they 

 are governed by the universal technique of bacteriology 

 which has heretofore been mentioned. ' 



The Bacillus Guillebeau is a facultative anaerobic 

 motile germ, gas forming, which does not liquefy gela- 

 tine. In stick cultures it grows needle-like with finely 

 pointed iris knobs. It causes sterilized milk to coagu- 

 late. Upon potatoes white or brown layers are formed. 

 According to Grame the bacillus does not stain. In 

 Guinea pigs and rabbits it is not pathogenic. Inoculated 

 into the udder it produces mastitis. 



The Staphylococcus mastitis Guillebeau, according 

 to Grame, is stainable and shows motility. It is faculta- 

 tive anaerobic, without gas development, liquefies gela- 

 tine with the formation of a sediment and causes steril- 

 ized milk to coagulate. Upon potato a yellowish white 

 layer is formed. The Streptococcus mastitis sporadicae 

 Guillebeau is also facultative anaerobic, liquefies gelatine 

 and thrives only very scantily upon potato. By inocula- 

 tion it shows no pathogenic peculiarities. 



