s Neurological Technique 



III. The Bethe Method. 



A special method for the differentiation of the neurofibrillae and 

 " Golgi net." As here given, the method is' applicable to vertebrate 

 nervous tissue 2$ 



IV. Apathy's " Aftergilding " Gold Chloride Method. 



An application of gold chloride to sections of material previously fixed. 

 A method for the neurofibrillae, and also adaptable to the study of 

 nerve terminations and the general morphology of the neurone 31 



V. KuPFFER's Method. 



A method for the study of the meduUated axone — the myelin sheath, 



the axis cylinder, the " nodes of Ranvier," etc. 37 



VI. A Silver Nitrate Method. 



An application of silver nitrate to the fresh nerve-fiber to demonstrate 



the so-called "cross of Ranvier," Frommann's lines, the neurolemma, etc. 41 



VII. The Intra-vitam Methylen-Blue Method. 



Applications of methylen-blue to the fresh tissue for the external mor- 

 phology of the neurone and to demonstrate the course and termina- 

 tions of the axone. Especially suited for the architecture of peripheral 

 ganglia and muscle-nerve terminations, but may be applied to the 

 central nervous system 43 



VIII. A Gold Chloride Method. 



Applications of gold chloride to either fresh tissue or to tissue which 

 has been fixed in formalin. Especially devised to stain muscle-nerve 

 terminations and peripheral plexuses t^i 



IX. The Golgi Method. 



A method for the external morphology of the neurone, central and 

 peripheral ; to demonstrate the shape of the cell-body, the dendrites and 

 their gemmules or "pin-head" processes, the axone and its branch- 

 ings, collaterals, and terminations. Also for neuroglia and for the 

 non-nervous tissue elements of the organs of special sense 55 



1. The " rapid method " 57 



2. Application to material fixed and preserved in formalin 60 



X. The Iron -Hematoxylin Method. 



A hsematoxylin method for nerve material preserved in formalin. 

 Differential for the medullary sheath and also stains the nerve-cell. 

 Especially adapted to the central nervous system to show the occur- 

 rence and course of meduUated fiber-tracts 63 



XI. The Weigert Method (Pal's Modification). 



A specific method for the medullary sheaths of the nerve-tracts of the 

 central nervous System. Applicable for the study of both the develop- 

 ment and extent of medullation as well as for tracing meduUated nerve 



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