METHOD I. 



AN ERYTHROSIN-TOLUIDIN BLUE METHOD.' 



A method for the general internal morphology of the neurone, 

 giving the granulation, " neurosomes," reticulum, and " axone 

 hillock." It is also differential for karyokinesis in the nervous 

 system. 



REAGENTS REQUIRED. 



A. Van Ge hue kten' s fixi?ig fluid. — 



Absolute alcohol, 60 cc. 

 Chloroform, 30 cc. 

 Glacial acetic acid, 10 cc. 



B. Contrast stain (alcoholic erythrosin). — 



Erythrosin (GrUbler's, soluble in alcohol), i g." 

 70 per cent alcohol, 100 cc. 



C. Stain (aqueous toluidin blue). — 



Toluidin blue (Griibler's), i g. 

 Distilled water, 100 cc. 



D. Differentiating fluid (o.i alum). — 



Potassium alum, 0.2 g. 

 Distilled water, 200 cc. 



E. Clearing fluid. — 



Oil of cedar wood, 25 cc. 

 Pure xylol, 25 cc. 



F. Meyer's albumen fixative. — 



White of egg (chop with scissors and filter), 50 cc. 

 Glycerine (pure), 50 cc. 

 Thymol, 0.2 g. 



G. Mounting medium. — A xylol or benzol solution of Canada 

 balsam, or, better, gum damar. Colophonium (see II, E, p. 21) 

 may be used. 



■ This method is a. modification based upon a method devised by H. Held, 

 "Beitrage zur Struktur der Nervenzellen und ihre Fortsatze,'' Archiv filr Anai.und 

 Physiol., Anat. Abthl., 1897. 



= g. = gram. 



15 



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