Method XIII 8i 



Arrange the sections on the slide in series, remove the sur- 

 plus clearing fluid with filter paper, and mount in balsam (X, 

 10, {c), p. 66), 



The axis cylinders should appear deep blue ; normal medul- 

 lary sheaths, yellowish red ; myelin globules of abnormal 

 sheaths, yellow ; nuclei, bluish red ; cytoplasm, bright red. 



For a description of the fibers in various stages of degenera- 

 tion and regeneration, the literature must be consulted. Stroebe's 

 paper of 1893' gives a complete list of the literature up to that 

 date, as well as a description of the appearances brought out by 

 his method. 



The method does not always succeed, and may require con- 

 siderable experiment before success is attained. Differentiate 

 and counterstain only a few sections at a time. If the material 

 is in more than one piece, prepare one piece at the time, leaving 

 the rest in the Miiller's fluid. 



" H. Stroebe, "Experimentelle Untersuchungen iiber Degeneration und Rege- 

 neration peripherer Nerven nacli Verletzungen," Beitr. zur path, Anai. u. zur allgem. 

 Pathol., Bd. 13, p. 160, 1893. 



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