METHOD XIV. 



WEIGERT'S METHOD FOR NEUROGLIA.' 



A differential stain for neuroglia, showing the relations 

 between the neuroglia fibers and neuroglia cells. Has given best 

 results with adult human tissue. 



SPECIAL REAGENTS REQUIRED. 



A. Fixing fluid (\0 per cent, formalin). — 

 For preparing, see X, A, p. 63. 



B. Chrom-alum solution. — 



Chrom-alum, 5 g. 



Distilled water, 200 cc. 



Acetic acid (C. P. conc), 10 cc. 



Neutral acetate of copper, 10 g. 

 The alum is first added to the water, and the water raised to the boiling 

 point. Then the acetic acid, and finally the copper acetate (pulverized) are 

 added. 



C . Potassium permanganate solution . — 



Potassium permanganate, i g. 

 Distilled water, 300 cc. 



D. Chromogen solutions. — 



1. Chromogen, 5 g. 

 Distilled water, 100 cc. 

 Formic acid (cone), 5 cc. 



When the chromogen has dissolved, filter the solution and to each 90 cc. 

 of the filtrate add lo cc. of a 10 per cent, aqueous solution of sodium sul- 

 phite. 



2. Chromogen, 5 g. 



Distilled water, loo cc. 



E. The stain. — 



Methyl violet, 2 g. 



70 per cent, alcohol, 100 cc. 



' C, Weigert, " Beitrage zur Kentniss der normalen menschlichen Neuroglia," 

 Abh. Senkenb. Ges., Frankfurt a. M., Bd. XIX, 1895. The slightly modified proce- 

 dure given here is taken largely from that followed by Aguerre : J. A. Aguerre, 

 " Menschlichen Neuroglia, tic," Archiv fur. mikros.Anai., Bd. 56, August, 1900. 



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