86 Neurological Technique 



section by its celloidin margin and carefully peel it off the slide 

 and place it in a covered dish containing 25 or 30 cc. of the 

 aniline-xylol mixture (H). This extracts the excess of the stain. 

 The optimum time required for this extraction varies. It may 

 require only 1 5 minutes or it may take y^ hour or more. No 

 final test of the decolorization can be made till the section can 

 be examined with a high-power lens. 



10. To mount. — Rinse the sections in pure xylol for a few 

 seconds, arrange them on the slide, and mount in balsam as in I, 

 16, p. 19. 



The neuroglia fibers can be satisfactorily studied only with an 

 oil immersion lens. Much can be seen, however, with a high- 

 power dry lens. 



The nuclei of the neuroglia cells show brownish blue and 

 neuroglia fibers appear pale blue. 



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