METHOD XV. 



MALLORY'S METHOD FOR NEUROGLIA.' 



An application of phosphotungstic acid-hjematoxylin as a 

 differential stain for neuroglia. A shorter method than Weigert's, 

 with the same purpose, and giving practically the same results. 



SPECIAL REAGENTS REQUIRED. 



A. Fixing fluids. — 



1. 10 per cent, formalin (X, A, p. 63), 200 cc. 



2. Saturated aqueous picric acid. 

 Picric acid crystals, i g. 

 Distilled water, 200 cc. 



Warm, shake repeatedly, let cool, and decant for use. 



B. 5 per cent. ammo?dum bichromate. — 



Bichromate of ammonium, 5 g. 

 Distilled water, 100 cc. 



C. o.^ per cent, potassium permanganate . — 



Potassium permanganate, i g. 

 Distilled water, 200 cc. 



D. / per cent, oxalic acid. — 



Oxalic acid crystals, I g. 

 Distilled water, 100 cc. 



E. The stain (phosphotungstic acid-haematoxylin). — 



HeEmatoxylin crystals, 0.2 g. 



Distilled water, 160 cc. 



Phosphotungstic acid, pure (Merck), 4 g. 



Distilled water, 40 cc. 



Peroxide of hydrogen (U. S. P.), 0.4 cc. 

 First dissolve the haematoxylin in the 1 60 cc. of water, then add it to the 

 40 cc. of 10 per cent, phosphotungstic acid also made up separately, and 

 finally, add the hydrogen peroxide. This results in about 200 cc. of the stain. 

 It keeps well. 



' F. B. Mallory, " A Contribution to Staining Methods," Journal of Experi- 

 mental Medicine, Vol. V, No. I, October, igoo. 



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