94 Neurological Technique 



chloride, and also, especially if glycerine is used, it is more 

 liable to crumble, the Miiller's fluid having made it brittle. 



If glycerine is to be used in the preparation, by all means use 

 it upon specimens which have been preserved in lo per cent, 

 formalin (X, A, p. 63). 



In the above procedures most of the shrinkage results from 

 the action of the alcohol in dehydration. After fixation in for- 

 malin the use of alcohol may be avoided entirely by simply 

 prolonging the time in glycerine. 



Fix and harden in 10 per cent, formalin for 2 weeks or 

 more ; transfer direct to pure glycerine, changed once, for 2-3 

 weeks, set out to " evaporate," and then shellac as before. This 

 will give a preparation of very nearly the normal dimensions. 



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