MORBID ANATOMY 



119 



Fowl No. 5or, fed culture March 26, 



Mar.26 



28 



Apr. 2 



3 



Number of 

 Tempera- red cor- 

 ture (F°) puscles 



per c. mm. 



Well. 



Fowl eats very little. 

 Blood very pale; fowl weak; refuses food. 

 Very weak; many red corpuscles at- 

 tacked by leucocytes. 

 Found dead. 



In fresh preparations of the blood of affected fowls exam- 

 ined in Toisson's fluid, red corpuscles which take the violet 

 stain more or less intensely 

 throughout are frequently 

 observed. 



In the blood of poultry 

 two distinct classes of white 

 corpuscles are conspicuous. 

 The first which predomin- 

 ates in numbers, contains 

 nuclei with from one to 

 four lobes, and the cyto- 

 plasm is sprinkled with a 

 variable number of round, 

 elongated, or spider-shaped 

 bodies. In the fresh con- 

 dition they are highly re- 

 fractory. They stain with 

 eosin, and if the prepara- 

 tions are heated suflBciently 

 they will retain certain of 

 the aniline dyes. The other class consists of round or nearly 

 round cells which takes the blue stain feebly. Usually it is 

 difficult to detect the nucleus, although it is occasionally dis- 

 tinct. Between these two types there are many varieties. 

 The leucocytes containing the spindle-shaped bodies appear to 

 be the phagocytes, as they were the only ones which were 

 observed to engulf the red corpuscles. Bacteria have not been 

 demonstrated in these cells, although their presence has, in 



Fig. 18. Section of chicken's liver 

 showing blood engorgement. 



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