lyo GLANDERS 



This film-like sediment may become so dense that it rolls in at 

 the periphery. The supernatant fluid becomes clear in the 

 lower dilutions, but in the higher ones the clarification may 

 not be complete, showing that all the bacteria have not 

 become agglutinated. This is further evidenced by the fact 

 that the layer is less dense . in the higher dilutions. The 

 reaction may begin in six hours, but cannot be considered 

 complete until 24 to 36 hours have elapsed. If no reaction 

 appears in 24 hours it cannot be considered negative, as it may 

 occur in from 30 to 40 hours after setting. Often, however, a 

 reaction appears in less than 24 hours. 



After the agglutination is completed, further standing 

 produced no visible change in the test fluid. 



A negative result shows a small round concentrated spot 

 of sediment in the center of the convexity at the bottom of the 

 tube, the test fluid remaining apparently unchanged even after 

 several weeks. Animals whose blood, serum agglutinates in 

 dilutions of 1-500 are suspicious and a reaction in dilutions of 

 1-800 or higher indicates an infection with Bad. mallei. 



As pointed out by Bonome and confirmed by Taylor, 

 there seems to be little or no change produced in the precipita- 

 ting power of the serum of the blood taken before, during or 

 after the mallein reaction but the agglutinating power as deter- 

 mined microscopically is very much increased during the 

 mallein reaction. 



This is shown' by the appended table : 



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