338 PNEUMONOMYCOSIS 



tures and examination of sections. Cultures were made on 

 glycerinated potato, bouillon and plain agar, by opening a 

 nodule with sterile instruments and tearing out a small portion 

 of the center, which was transferred to the culture tubes and 

 placed in an incubator at 39° C. Abundant growth was ob- 

 tained on the potato by the end of thirty- six hours, white at 

 first but soon changing to a yellowish and later to a dark green 

 color. The growth in the bouillon and agar was slow. Plates 

 and flasks of bread paste were made, and these with potato 

 were employed for all subsequent cultures. The formation of 

 the fruit hyphae was studied. The spores measured from 2.5 

 to 3.5/U in diameter. By these means the culture was identified 

 as the aspergillus fumigatus. The experiments on other 

 animals were limited to the inoculation of one rabbit, into the 

 aural vein of which one-half cubic centimeter of a suspension 

 of the spores was injected. The animal died in forty-four 

 hours and from the liver and kidneys cultures were recovered. 

 All of the organs were examined in sections, but the mycelium 

 was detected in two only. 



Microscopic examination. The nodules were hardened and 

 embedded, some in collodion and some in paraffin. Various 

 staining methods were tried, hematoxylin and eosin, carbol- 

 thionine. Gram and lithium carmine with Weigert's fibrin 

 stain. Good results were obtained with all, but the carmine 

 and Weigert gave the most beautiful picture and by this 

 method the fungus was most perfectly demonstrated, the spores 

 and mycelium taking on a deep purple color. The histology 

 was studied largely in sections stained with hematoxylin and 

 eosin. The bronchial epithelium was normal in places, but, 

 for the most part, the columnar cells had been replaced by a 

 sort of membrane, which appears to be made up almost entirely 

 of a felt-work of mycelial threads. From this membrane 

 hyph« grew out into the lumen of the bronchus, and here, 

 owing no doubt to the supply of air, fruit hyphae arose, with 

 perfect sterigmata and spores. There was no cellular nor other 

 exudate and very little debris. The under surface of this 

 membrane was of looser texture and contained some cellular 



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