CONTENTS. 



PAGE 



Introduction with Historical Sketch ... i 



PART I. 

 BACTERIOLOGICAL TECHNIC. 



CHAPTER 1. 



Examination of Bacteria with the Microscope, Including Methods of 



Staining . ... i8 



CHAPTER II. 

 Sterilization . . . 53 



CHAPTER III. 

 Culture-media . . . . 64 



CHAPTER IV. 



The Cultivation of Bacteria. Tube-cultures; the Incubator; Anaerobic 



Methods. . . .... . 78 



CHAPTER V. 



The Cultivation of Bacteria (Continued). Isolation of Bacteria; Plate- 

 cultures ' . . 94 



CHAPTER VI. 

 Inoculation of Animals. Autopsies; Collodion Sacs 102 



CHAPTER VII. 

 Collection of Material 107 



CHAPTER VIII. 



Systematic Study of Species of Bacteria. Suggestions for Class-work; 



Rules 112 



ix 



