PATHOGENIC BACTERIA. 365 



from the nose. The neighboring lymph-nodes become 

 involved and are swollen, and nodules may be present in the 

 internal viscera. In the skin the nodes lying underneath the 

 skin are called farcy-buds. Histologically the nodules con- 

 sist of a granulation tissue, but they tend to break down 

 rapidly, and the process in some respects is very like ordinary 

 suppuration. 



In addition to the spontaneous infection of horses and 

 asses, cultures are pathogenic* for guinea-pigs, European 

 field-mice and cats; rabbits, sheep and dogs are less susceptible 

 or only slightly so; also white and house- mice, and hogs; 

 cattle are immune. Camels are susceptible, hedgehogs also. 

 Men are occasionally infected, especially those who come much 

 in 'contact with horses. The mucous membranes of the nasal 

 cavity may be the part involved, or the skin or the internal 

 viscera. In a number of instances, workers in the laboratory 

 have been accidentally infected. 



The diagnosis of the disease is best effected by the inocu- 

 lation of a male guinea-pig with the material from a case sus- 

 pected of being glanders, introducing it into the peritoneal 

 cavity (method of Straus). Frothinghamf describes the 

 method of procedure which he employs as follows : 



The material from the suspected animal — nasal secretion or 



skin lesion or both — is obtained by using a swab such as is 



used to obtain material for diagnosis in diphtheria. After 



it has been applied to the lesion the cotton plegget is removed 



to a convenient quantity — 2 or 3 c.c. — of sterile water, and 



agitated. The water is then injected in equal portions into 



the peritoneal cavity of two guinea-pigs. In about two to 



three days after an inoculation of this kind there appears a 



characteristic swelling of the testicle, indicating the beginning 



of suppuration, which presently takes place; the animal usually 



*The statements of different writers differ considerably with regard to some 

 of these animals. 



fJourn. Med. Research. Vol. VI. 1901. pp. 331-340. 



