436 MANUAL OF BACTERIOLOGY. 



18 . Best media for long-continued growth 



19. Quick tests for differential purposes 



III. PHYSICAL AND BIOCHEMICAL FEATURES. 



I. Fermentation-Tubes containing peptone- 

 water or Sugar-free bouillion and 



Gas production, in per cent. 





Growth in closed arm 



Mill 



Amount of acid produced id. 



Amount of acid produced 2d. 



Amount of acid produced 4d. 



2 . Ammonia production, feeble, moderate, strong, absent, masked 

 by acids. 



3. Nitrates in nitrate broth. 

 Reduced, not reduced. 



Presence of nitrites ammonia 



Presence of nitrates free nitrogen 



4. Indol production, feeble, moderate, strong. 



5. Toleration of Acids: great, medium, slight. 

 Acids tested 



6 . Toleration of Na O H : great, medium, slight. 



7 . Optimum reaction for growth in bouillon, stated in terms of 



Fuller's scale 



8. Vitality on culture media: brief, moderate, long. 



9. Temperature relations: 



Thermal death-point (10 minutes exposure in nutrient broth when 



this adapted to growth of organism) C. 



Optimum temperature for growth C.; or best growth 



at 15° C, 20° C, 25° C, 30° C, 37° C, 40° C, 50° C, 60° C. 



Maximum temperature for growth C. 



Minimum temperature for growth C. 



10. Killed readily by drying: resistant to drying. 



11 . Per cent, killed by freezing (salt and crushed ice or liquid air) 



12. Sunlight: Exposure on ice in thinly sown agar plates: one -half 

 plate covered (time 15 minutes), sensitive, not sensitive. 



Per cent, killed 



