CONTENTS 



CHAPTER I 

 THE PREPARATION OF SPECIMENS 



PAGE 



Sectioning Methods 1 



Infiltration Method 2 



Fixation 2 



Dehydration 3 



Clearing 4 



Infiltration 5 



Embedding 6 



Section Cutting, Hand and Machine Microtomes 7 



Ribbon Sectioning 13 



Removal of Embedding Media 14 



Embedding Method for Non-infiltrated Specimens 15 



Fresh Materials 15 



Dried Materials 16 



Blocking for Cutting 16 



Synopsis of Sectioning Methods 17 



CHAPTER II 

 THE MOUNTING OF SPECIMENS 



Mounting Media 20 



Preparation of Glycerin Jelly Mounts 21 



Preparation of Canada Balsam Mounts 23 



CHAPTER III 



THE MICROSCOPE 



The Optics of the Microscope 24 



Refraction 24 



Types of Lenses 25 



Optic Axis 26 



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