226 APPENDIX 



Cleaeing Agents: 



Clove, Lemon, Turpentine, Bergamot and Sassafras Oils, 



Liquefied Phenol, 



Xylol, 



Chloroform, 



Canada Balsam in Xylol. 



Cuprammonia, (Ammoniacal Copper) (Schweitzer's Reagent) 

 (Cuoxam) : 

 Formula A 



Copper Sulphate 15 per cent aqueous solution 



Sodium Hydroxide 3 per cent aqueous solution 



Add slight excess of the hydroxide solution to the copper sulphate 

 solution. Wash the precipitate by decantation and dissolve it in con- 

 centrated ammonia water. 



Formula B 



Cover small pieces of metallic copper with 15 per cent 

 ammonia water. Allow the mixture to stand in an open 

 vessel for several days. Filter through glass wool. 



Cupric Acetate: 



Cupric Acetate 15 grams 



Distilled Water, to make 100 mils 



Delapield's Hematoxylin: 



Hematoxylin 4 grams 



Alcohol 25 mils 



Ammonia Alum 50 grams 



Glycerin 100 mils 



Methyl Alcohol 100 mils 



Distilled Water 400 mils 



Dissolve the hematoxylin in the alcohol and the alum in the water. 

 Mix these solutions and allow to stand exposed to light and air for 

 five days. Filter, add the glycerin and methyl alcohol and allow to 

 stand exposed to light for five days. Finally filter and store for use. 

 This stain improves upon keeping. 



Fehling's Solution (Alkaline Cupric Tartrate) : 



Alkaline Tartrate Half. 



Rochelle Salt 17.3 grams 



Sodium Hydroxide 5.0 grams 



Distilled Water, to make 50.0 mils 



