VI CONTENTS. 



CHAPTER VI. 



PAGE 



Preparation of media.— Bouillon, gelatin, agar-agar, 

 potato, blood-serum, etc. . . ... 59-76 



CHAPTER VII. 



Preparation of the tubes, flasks, etc., in which the media 

 are to be preserved . • 77-80 



CHAPTER VIII. 



Technique of making plates — Esmarcli tubes, Petri 

 plates, etc. . • 81-90 



CHAPTER IX. 



The incubator used in bacteriological work — Gas-press- 

 ure regulator — Thermo-regulator — ^The form of burner 

 employed in heating the incubator . . . 91-98 



CHAPTER X. 



The study of colonies— Their naked-eye peculiarities 

 and their appearance under different conditions — Differ- 

 ences in the structure of colonies from different species of 

 bacteria — Stab cultures — Slant cultures . . 99-103 



CHAPTER XI. 



Systematic study of an organism — Steps necessary in 

 identifying an organism as a definite species . . 104-121 



CHAPTER XII. 



Methods of staining — Solutions employed — Preparation 

 and staining of cover-slips —Preparation of tissues for 

 section-cutting— Staining of tissues — Special staining 

 methods 1-22-150 



CHAPTER XIII. 



Inoculation of animals — Subcutaneous inoculation — 

 Intra-venous injection . 151-158 



