260 



INDEX. 



Bacteria, reactions produced by, 

 114 

 relation to oxygen, 27, 28 

 to staining reagents, 115 

 to tempei-ature, 28 

 role in nature, 23-25 

 systematic study of, 104-121 

 scheme for, 164, 165 

 Bacterium coli commune, 230, 231 

 Benches, glass, for holding plates, 



85 

 Blood-serum, 69 



Loffler's mixture, 75, 76 

 preparation of, 69 

 preservation of, 73, 74 

 solidification of, 71-73 

 sterilization of, 71 

 Bonnet, 20 



Bottles for staining solutions, 128 

 Bouillon, 57-59 



neutralization of, 60, 61 

 Box, iron, in which to sterilize 



plates, 84 

 Brownian motion, 110 

 Bulb for water samples, 179 

 Burner, Koch's safety, 93, 94 

 rose or crown, 47 



riHLOEOPHYLL, definition 

 \J of, 22 

 Cohn, 20 



Colonies, characteristic appear- 

 ance of, 168, 169 

 study of, 99-101 

 Cooling-stage, 84 

 Cover-slips, cleaning of, 123 

 impression, 126 

 microscopic examination of, 



107 

 preparation of, 123-127 

 steps in making, 124 

 Culture-dish for plates, 85 

 Cultures, gelatin, 113 

 hanging-drop, 109 

 potato, 113 



in tube, 69 

 pure, 101 

 stab and smear, lOl-lOo 



method of making, 101, 

 102 



DECOLORIZING solutions, 189 

 Decomposition, 22, 23 

 Diphtheria, 243 



bacillus of, 245-252 



cultivation of, 245-248 

 pathogenic properties of, 



249-252 

 preparation of cultures 



from, 243-245 

 staining of, 248 

 Diplococoi, 30 



Diplococcus of pneumonia, 194 

 Disinfectants, experiments upon, 



253-257 

 Disinfection, 49-53 



agents employed for, 52, 53 

 in the laboratory, 5'2 

 inorganic salts for, 49-51 

 methods of, 52 



I7EYSIPELAS, 224 



Ju Esmarch's roll tubes, 87 



modification of Esmarch's 

 method, 88 

 advantages of this pro- 

 cess, 89 

 roll tubes of agar-agar, 89 

 tube being rolled on ice, 88 

 Experiments, 167 



exposure and contact, 169, 

 170 



PA(JULTATIVE aerobic and 

 I anaerobic organisms, 28 

 Fermentation, 22, 23, 116 

 Filters, preparation of, 64 

 Flagella;, Lofilei-'s method of 



staining, 136 

 Flasks, etc., cleaning of, 77 

 P\mnel for filling Sedgwick's, 

 aerobioscope, 190 

 for filling test-tubes, etc., 79 



p ELATIN, 57-62 

 \J clarification of, 63 



filtration of, 63 



peculiarities of, 57, 58 

 Guarniari's agar-gelatin, 76 



