STAINING OF PNEUMONIC LUNG. 29 



(d) Rinse in water, dry and mount in Canada 

 balsam. 



4, Prepare cover glass films from the sputum, (pre- 

 served in 5% carbolic acid), of a patient with Influenza 

 Bronchitis. Dry and pass through the flame. Clear 

 for 1 to 2 minutes in the alcohol-ether mixture. 



Stain in Loeffler's methylene blue or in anilin gen- 

 tian violet in the usual manner. 



The Influenza bacilli appear as fine short rods, at 

 times forming fine segmented threads. Many lie in pus 

 cells. 



Staining of Pneumonic Lung (red hepatization), 

 for Fibrin and Pneumococci. 



5. Owing to the fragile nature of the sections, the 

 staining processes are best carried out on a slide. The 

 use of such fixing fluids as the glycerin albumin mix- 

 ture, should be avoided, owing to the precipitation of 

 the stains. The sections are given out in alcohol. 



(a) With a section lifter remove a section to a slide, 

 and remove excess of alcohol with blotting paper. 



(b) Cover the specimen with anilin gentian violet for 

 15 minutes, protecting from dust by placing under a 

 bell jar. 



(c) Remove the stain by blotting paper and carefully 

 wash with water. 



(d) Treat the sections with Weigert's iodine for 2 to 

 3 minutes. 



(e) Remove the iodine solution and wash in water. 



