42 PRACTICAL BACTERIOLOGY. 



DEMONSTRATION XVI. 



Examination of animal inoculated with Spirillum Choleras Asiaticae — 



Cultures of Bacillus Typhosus and Bacillus Coli 



Communis — Typhoid Tissues. 



Examination of animal inoculated with the Spirillum 

 of Cholera. 



Open up the abdominal cavity of the guinea pig, 

 using all aseptic precautions (page 20). 



Note the condition of the peritoneum and character 

 of the exudate. 



1. With a sterile loop, or capillary pipette, transfer 

 a drop of the peritoneal exudate to a liquefied gelatine 

 tube. From this tube inoculate a second. 



Pour plates in the usual manner. 

 Incubate at 20°C. and examine carefully on succes- 

 sive days. 



2. With a sterilized loop, smear a series of cover 

 glasses, with the peritoneal exudate. 



Stain some films with eosin and methylene blue; 

 others may be stained with anilin gentian violet or 

 carbol fuchsin after clearing in 10% acetic acid. 



3. Carefully open the thorax, sterilize a portion of 

 the exposed heart surface, xand with a capillary pipette, 

 withdraw blood from the chambers. 



From this blood make cover glass preparations, fix 

 in formalin solution, and stain with eosin and methylene 

 blue. 



