BACILLUS PESTIS BUBONICE. 47 



DEMONSTRATION XVIII. 



Bacillus Pestis Bubonica- — Bacillus Icteroides — Bacillus Mallei — 



Cultures of Tricophyton Tonsurans and Achorion 



Schonleinii —Hair in Tinea Tonsurans. 



1. Prepare cover glass films from the agar cultures 

 of Bacillus pestis buborticae, Bacillus icteroides and 

 Bacillus mallei. Stain in anilin gentian violet or in 

 anilin fuchsin. 



2. Remove in the usual manner a small portion of 

 the growth of Tricophyton tonsurans from the cultures 

 furnished. Tease out on a slide after treatment with 

 10% caustic potash solution. Examine with the low 

 and high dry lenses. 



3. In like manner examine a preparation from the 

 culture of Achorion Schonleinii (Favus). 



Compare this fungus with Tricophyton tonsurans. 



4. 'Treat in the same manner the hairs furnished 

 from Tinea tonsurans. The fungus threads and spores 

 are seen lying in the root sheath and also in the hair 

 shaft. 



This method of ^examination may be adopted for the 

 detection of the fungus elements in Ringworm else- 

 where (Tinea barbae, Tinea circinatse), and also in 

 Favus and Tinea versicolor. 



