20 PRACTICAL BACTEEIOLOGY. 



DEMONSTRATION VIII. 



Examination of guinea pig dead of Anthrax Septicaemia— Plate 

 cultivations— Cultures of the Pyogenic Bacteria. 



Guinea Pig dead of Anthrax. 



1. (a) Carefully examine the animal, noting the 

 swelling extending from the groin, to a variable extent 

 over the abdomen. Then stretch out the animal by 

 tacking down the legs to a board, moistened with 

 1: 1000 bichloride of mercury solution. 



(6) Lay the hair of the animal by moistening with 

 methylated spirit. 



(o) With a sterilized scissors carefully cut through 

 the skin along the median line and reflect it from over 

 the thoraxj abdomen and thighs. 



(d) Note the situation and character of the oedema. 

 From the cedema fluid, smear cover glass films for after 

 staining. (Cultures might also be made.) 



(e) Sterilize the abdominal wall in the midline and 

 the thorax at the rib-costal junctions, with a flat-bladed 

 knife, heated to redness. 



(/) With sterilized forceps and scissors open into 

 the abdomen along the midline. With fresh forceps 

 remove the spleen and break a portion of it up in a 

 sterile capsule. From the broken pulp, make cover 

 glass preparations by placing a drop of pulp on one 

 cover glass and dropping a second cover glass upon this, 



