18 PRACTICAL BACTERIOLOGY. 



(d) Immediately wash in fresh water. 



(e) Dehydrate in absolute alcohol for 1 to 2 minutes. 

 (/) Clear in xylol for 1 to 2 minutes. 



(g) Transfer to slide with section lifter and mount 

 in Canada balsam. 



Sections after dehydration may be transferred to 

 slide and cleared there with xylol before mounting, as 

 xylol causes at times considerable shrinkage and 

 curling. 



Examine with the low and high dry lenses and the 

 T 1 ¥ oil immersion. Anthrax bacilli are seen in the 

 capillaries of the organs. 



5. Staining by Gram's method and eosin. 



(a) Place the sections in alcohol for 1 minute. 



(6) Transfer to anilin gentian violet, 5 to 10 minutes. 



(c) Rinse the sections in distilled water, and then 

 place for 2 minutes in Gram's iodine solution. 



(d) Rinse in water, and then in alcohol for \ minute. 



(e) Place for 1 minute in eosin staining fluid, 



Alcoholic eosin ... .5 gramme. 



Alcohol 70 cc. 



Water 30 cc. 



(/) Remove excess of eosin by washing in water and 

 then in alcohol. 



(g) Transfer to acid alcohol for 5 to 10 seconds, and 

 again wash in alcohol till the sections become red. 



(h) Clear in xylol, transfer to slide, and mount in 

 Canada balsam. 



