HANGING DROP CULTURES. 11 



in like manner, and covered by a- bell jar, sterilized by 

 washing in 1 : 1000 bichloride of mercury. 



(b) With a sterile loop carefully place drops of 

 broth (sterile) on the cover glasses. 



(c) Lightly inoculate these drops with the respective 

 bacteria. 



(d) Invert these drops over the cell of a hollow 

 ground slide, previously sterilized, and having edge of 

 cell ringed with vaseline. 



(e) Place in the incubator at 37°C, on slide rack. 



