PREFACE. 



This book has been prepared for the guidance of 

 students taking the practical course in Bacteriology. 

 Much time and labor have hitherto been expended in 

 the writing out, both by the lecturer and the student, 

 of the daily work of the class, and to obviate this it 

 was thought best to place these notes in an accessible 

 and connected form. The usual course covers a period 

 of from eight to ten weeks, and comprises a series of 

 twenty demonstrations, of at least two hours each. 



There are already several excellent handbooks for 

 the guidance of students, notably that of Kanthack 

 and Drysdale. As the author worked under the for- 

 mer's guidance, their methods have been used as a 

 basis. Free use has also been made of methods advised 

 by, or acquired from others, which have been found 

 satisfactory. 



In Part II the preparation of culture media, methods 

 of sterilization of bacteriological utensils, and the 

 methods followed in water and milk analysis are 

 considered. 



The author has appended in Part III the plan of 

 work followed in the usual course in clinical microscopy 

 and diagnosis, covering that elementary work, with 

 which every physician should now not only be cogni- 

 zant, but employ. 



W. T. Connell. 



Kingston, Ontario, 

 May 1st, 1899. 



