414 DISEASES LOCALIZED IN CERTAIN ORGANS 



is to inject the "lymph" intravenously. The danger of 

 phlebitis, however, makes its use rather hazardous. 



(c) Old Method. — ^The material was collected by expressing 

 from the interlobular connective tissue of a hepatized area 

 of an affected lung a quantity of the liquid exudate which was 

 filtered through clean linen to remove solid matter. The 

 injection was made under the tail tip after the hair had been 

 shaved from an area and the skin disinfected. A more 

 primitive method was to soak a piece of woolen thread in the 

 exudate and insert it as a seton under the skin. In one week 

 to one month a swelling occurred at the point of inoculation 

 and the animal would show slight fever. If the material 

 used was contaminated or infection allowed to enter the 

 inoculation wound, necrosis of the tail and sometimes even 

 pyemia result. The mortality from this method is about 

 2 per cent, and about 10 per cent, suffered loss of tail. 



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