Immunization. 



45 



We injected 2 c.c. of the serum of an affected horse, 

 which was gradually increased up to 2000-4000 c.c. with 

 a pause of 3 to 6 days. Only three of the seven horses 

 were made perfectly immune, while two died of infec- 

 tious anemia during the course of the treatment, and 

 with the remaining two it was necessary to stop the 

 treatment, because of the swellings and hyperaesthesia 

 at the site of inoculation. The protocol of one of the 

 perfectly immunized horses is given here. 



Immunized horse (extra series No. 1.) 



Aug. 7 (1909) virulent serum A 2.0 c.c. 



10 

 13 

 16 

 25 

 28 

 31 



Sept. 3 

 6 

 11 

 18 

 21 

 24 

 27 

 30 



Oct. 3 

 6 

 9 

 12 

 16 

 19 

 28 



Nov. 12 



4.0 



8.0 



16.0 



30.0 



60.0 



120.0 



„ E 240.0 



„ C 480.0 



„ „ 980.0 



virulent blood C 10.0 



„ „ 20.0 



B. 

 C. 

 D. 





F. 

 B. 

 G. 

 H. 

 I. 

 J. 

 B. 



40.0 



80.0 



150.0 



300.0 



500.0 



400.0 



500.0 



1000.0 



1500.0 



2000.0 



blood was 



From August 17 

 to 20 the tem- 

 perature rose to 

 40.S°C., mucous 

 membrane was 

 yellowish, and 

 petechiae were 

 seen. Inocula- 

 tion was, there- 

 fore, suspended 

 for a while. 



drawn. 



