Preventive Inoculation and Disinfection. 47 



not be used for therapeutic purposes; for it has no in- 

 fluence upon the virus. Moreover, the actual appHcation 

 in many cases has shown that it has no beneficial effect 

 upon the temperature and other general symptoms. 



It should especially be mentioned here that isolysin 

 is found in the immune serum, if the blood corpuscles 

 have not been excluded from the material used as anti- 

 gen ; for the patient that had been treated with such 

 serum suffered from a severe case of anemia on the 

 day following inoculation. A similar effect was produced 

 upon a healthy horse, in which the erythrocyte-count 

 fell suddenly from 8,000,000 to 2,700,000 on the day 

 following injection. 



XL PREVENTIVE INOCULATION AND 

 DISINFECTION. 



From what has already been described, it is certain 

 that the horse may be made immune against infectious 

 anemia, and we made a number of experiments to effect 

 the preventive inoculation. The results of all these ex- 

 periments, however, turned out to be negative. They 

 will be briefly described in this report not because of 

 their having had any practical value, but because they 

 contain some interesting facts concerning the nature of 

 the disease. 



A. Experimental Preventive Inoculation with 

 the Sterilized Virus. 



The virulent serum that had been sterilized at 60°C. 

 for one hour was inoculated into healthy horses. After 



