48 Preventive Inoculation and Disinfection. 



a certain pause, some of them were again inoculated 

 with the virus and they all became ill. 



B. Experimental Preventive Inoculation 



with the Attenuated and the 



Modified Virus. 



1. A goat was inoculated continuously for months 

 with the virulent serum. Its blood was drawn on the 

 10th-15th day from the last inoculation and the serum 

 was prepared. The serum was then inoculated into 

 healthy horses to see if it had any preventive efficacy 

 upon the disease. 



Experimental goat No. 1. received the virulent serum 

 subcutaneously for four days (from August 28, 1910) 

 and again for six days (from September 4, 1910), the 

 quantity of the last inoculation being 100.0 c.c. The 

 blood was drawn on the eleventh day from the last 

 inoculation. 



Experimental goat No. 2. received four subcutaneous 

 inoculations with the virulent serum in the course of 

 the period extending from August 28 to November 11, 

 the last dose being 50.0 c.c. The blood was drawn on 

 the 13th day. 



Experimental goat No. 3 received three subcutaneous 

 inoculations of the virulent blood, the last dose being 

 40.0 c.c. The blood was drawn on the 13th day. 



Experimental horse No. 95 received intravenous ino- 

 culation of 50.0 c.c. of the blood of Experimental goat 

 No. 1. No change took place for 33 days. It was then 

 inoculated with 20.0 c.c. of the virulent serum, and at 

 last, it contracted the disease with a period of incubation 

 of 11 days. 



