50 Preventive Inooolation aku Disinfection. 



the calf's serum subcutaneously. After 14 days, it again 

 received 20.0 c.c. of the virulent serum under the skin. 

 With a period of incubation of 12 days it became ill. 



Experimental horse No. 452 received 40.0 c.c. of the 

 calf's serum under the skin. After 40 days it was in- 

 oculated with 50.0 c.c. of the virulent serum. Symptoms 

 of the disease appeared on the 17th day. 



Experimental horse No. 398 received 30.0 c.c. of the 

 calf's bile at two places. The sites of inoculation became 

 swollen and suppurated. It received 20.0 c.c. of the 

 virulent blood after 14 days, and it became ill with a 

 period of incubation of 18 days. 



Experimental horse No. 445 received 20.0 c.c. of the 

 calf's bile at two places. Swellings were noticed at 

 the sites of inoculation, but they were soon absorbed 

 without suppuration. After 60 days the horse received 

 50.0 c.c. of the virulent serum. With an incubation 

 period of 21 days it contracted the disease. 



C, Experimental Preventive Inoculation 

 with the Immune Serum. 



The immune serum was made sterile either by adding 

 0.5 9^ of tricresol solution or by heating at 60°C. for 

 one hour, and its prophylactic efficacy was tested in the 

 following manner. 50.0 c.c. of the serum was inoculated 

 into horses once a month through the entire period in 

 which they were kept in the same pasture with the 

 patients. The horse (No. 468) that was treated with 

 tricresol serum became ill after 96 days. One of the 

 two horses (No. 488) that received the heated serum 

 contracted the disease after 152 days, while the other 

 (No. 480) remained healthy. 



