Draft of Law foe the Pkevention of Infectious Anemia. 51 



D. Disinfection. 



1. Carbolic acid. — In order to determine their 

 efficacy in killing the virus, several disinfectants were 

 experimented with by adding a certain quantity of each 

 to the virulent serum, which was then injected into 

 normal horses. Since we found out that constant results 

 could never be obtained, for the reason that the quantity 

 of the virus in the serum might vary with each case, 

 we used in our experiment, a large quantity of the serum 

 to reduce this variation. 4^ carbolic acid solution was 

 mixed in equal parts with 40-50 c.c. of the serum, so 

 that the efficacy of the mixture might be equal to the 

 2% solution of the disinfectant. The results showed that 

 the 2o/o carbolic acid solution did not kill the virus in 

 15 minutes. In 30 minutes only an incomplete action 

 sets in. In one hour it destroyed all the virus; for no 

 symptoms were produced in the inoculated horse. 



2. Chloroform and toluol.— These disinfectants were 

 added in various quantities to the virulent serum and 

 thoroughly shaken up. After 20 hours' standing the 

 mixture was inoculated into experimental horses, which 

 were all affected with typical symptoms. From this the 

 conclusion may be drawn, that chloroform and toluol do 

 not destroy the virus of infectious anemia. 



XII. DRAFT OF LAW FOR THE PREVENTION 

 OF INFECTIOUS ANEMIA. 



The Committee wished to apply to the prevention 

 of infectious anemia among horses part of the Laws 

 for the Prevention of Infectious Diseases of Domestic 



