112 BIOLOGY AND TECHNIQUE 



Decolorize with anilin oil until no more color comes off. 



This both decolorizes and dehydrates. 



Treat with xylol. Mount in balsam. 



Method op Staining fob Tubercle Bacilli in Sections.' — 

 Paraffin Sections. — Stain in carbol-fuchsin solution hot for five minutes 

 (or better cold, for twenty-four hours) . 



Wash in water. 



Decolorize and counterstain in Gabbet's methylene-blue sulphuric 

 acid mixture for one minute. 



Wash in water. 



Dehydrate in absolute alcohol. 



Clear in xylol. 



Mount in balsam. 



Celloidin Sections}— Sta,m lightly in alum hematoxylin. 



Wash in water. 



Dehydrate in ninety-five per cent alcohol. 



Attach the slide by ether vapor. 



Stain with steaming carbol-fuchsin two to five minutes. 



Wash in water. 



Wash with Orth's acid alcohol (alcohol ninety per cent., 99 c.c; 

 cone. HCl, 1 c.c.) one-half to one minute. 



Wash in water several changes. 



Treat with ninety-five per cent alcohol until red color is entirely 

 gone. 



Blot and cover with xylol until clear. Mount in balsam. 



Method of Staining Actinomyces in Sections. — Mallory's Method?. 

 — 1. Stain deeply in saturated aqueous eosin ten minutes. 



2. Wash in water. 



3. AniHn gentian-violet two to five minutes. 



4. Wash in normal saline solution. 



5. Weigert's iodin solution (iodin 1, KI 2, and water 100 parts) 

 one minute. 



6. Wash in water and blot. 



7. Clear in anilin oil. 



8. Xylol several changes. 



9. Mount in balsam. 



1 Mallory and Wright, " Pathol. Tech.," p. 413. 



2 After Mallory and Wright. 



» Mallory and Wright, " Pathol. Tech.," 1904. 



