654 



DISEASES OF UNKNOWN ETIOLOGY 



which is regulated so as to vary little above 25° C. Bacteriological 

 controls as to the sterility of the cord should also be made. 



After a suitable period of drying, pieces of the cord are prepared 

 for injection. This is done in various ways at different laboratories. 

 No attempt at exact dosage is made. At the New York Depart- 

 ment of Health 1 cm. of the cord is emulsified in 3 c.c. of sterile 

 eight-tenths per cent salt solution, the dose for injection being usu- 

 ally 2.5 c.c. Marx* emulsifies 1 cm. of the cord in 5 c.c. of sterile 

 bouillon or salt solution, using 1 to 3 c.c. of this for injection according 

 to the age of the cord. For shipment an addition of 20 per cent of 

 glycerin and 0.5 per cent of carbolic acid is made. 



The scheme of treatment is also subject to variations according to 

 the individual customs of various laboratories. The following scheme 

 is the routine of the Pasteur Institute in Paris, as quoted in Kraus 

 and Levaditi, "Handbuch fiir Immunitatsforschung," Vol. I, p. 713. 



^Marx, Deut, med. Woch., 1899, 1900. 



