112 GLANDERS 
known as the Strauss method. Hallopian and Bureau observed an 
orchitis following the inoculation of pus from a case of human mycosis 
into the peritoneal cavity of a guinea pig. Nocard recorded nineteen 
cases of a slightly contagious, farcy-like lymphangitis in horses due to 
a bacterium which produced an orchitis when inoculated into guinea 
pigs but which was different from Bact. mallet both in its cultural 
characteristics and its reaction to the Gram stain (Benson). The 
inoculation of the guinea pig in making a diagnosis can be consid- 
Fic. 9. THE SO-CALLED “GLANDERS EXPRESSION.” 
ered as only one factor. The bacterium must. be obtained from the 
lesions and identified. Infection takes place through the digestive 
tract. Experimentally it has been induced through the respiratory 
organs. 
The period of incubation is not definitely known. It varies from a 
few to many days, depending upon the method of infection and 
the virulence of the organisms, as well as the resistance of the animal. 
Symptoms. Two forms of glanders have been recognized, namely, 
acute and chronic. 
Acute glanders. Acute glanders is common in the ass and mule, but 
Jess frequent in the horse. After a short period of incubation the 
animal has a chill, elevation of temperature, a profuse muco-purulent, 
