138 GLANDERS 
which does nét prevent hemolysis contains a dilution of 1-80 (tube 5, Table V), then 
a dilution of 1-160 of the extract is used for the glanders test. Antigen which possesses 
too much anti-complementary substance should not be used; that is, where hemolysis 
is prevented in as high dilutions as 1-300, 1-400, 1-500, etc. 
Suspected serum. The suspected glandered horse is bled usually through the jugular 
and about 20 ce. of the blood is collected in a small bottle. The blood is then allowed 
to coagulate and the serum to collect. If the blood came from an animal affected with 
glanders, the serum contains the specific glanders bacteriolytic amboceptor or immune 
bodies. The amount of these immune bodies in the blood of glandered horses is not 
TABLE No. V 
TITRATION OF GLANDERS BACTERIA EXTRACT, 
Tube | NaCl so- | Comple- Ambocep-|Blood cor 
No. | lution (a) | ment (b) Extract (c) tor (d) |puscles (e) 
1 lee. lec. | 1lec.of 1-10 | lee. 1 ce. 
2 lee. lee. | lee.of 1-30 | lce. l ce. 
3 lee. lee. | lec.of 1-50 | 1 ce. l ce. 
4 lee. lee. | lec.of 1-60 | 1 ce. 1 ee. 
5 lce. lee. | 1lec.of 1-80 | Ilce. lee. 
6 lce. lee. | 1lec.of 1-100) 1 ce. 1 ce. 
7 lee. lee. | lec. of 1-200] 1 cc. lee. 
8 lee. lee. | lec. of 1-250} 1 ce. 1 ce. 
9 1 ce. lee. | lee. of 1-300} 1 cc. l ce. 
10 lee. lec. | lec. of 1-400} 1 ce. lee. 
11 lee lee. | 1lec.of 1-500} 1 ce. lee. 
12 3 ec. lee controls lec. | Comp. control (no hem- 
olysis should occur) 
18 3 ce. lee. lec. | Ambocep. control (no 
hemolysis should occur) 
14 4 ce. lec. | Salt solu. control (no 
hemolysis should occur) 
NaC] solution. 
The determined smallest quantity established according to the primary test. 
Dilutions made according to Table IV. 
Double the quantity previously determined by titration. 
5% suspension of washed sheep blood corpuscles. 
teore 
uniform and probably depends to some extent on the degree of infection present, there- 
fore it is advisable to use in the test such quantities of the serum as will prove sufficient 
for the reaction to take place. 
