GLANDERS 139 
TABLE No. VI 
THE FINAL TEST FOR GLANDERS. 
Glan- == a 
Tube} NaCl ee ders. | Com | Ambo-| Blood 
No. as heres bacteria/plement| ceptor |corpus- R ke 
on 1 | ram 2 extract 4 6 cles 6 emarks 
1] ce. | ec. ec. ce. cee. ee. | Test tube for the dose 0.1 cc. of 
1 | 0.1 1 1* 1 1 suspected serum 
2 2 | OM liso 1 1 1 | Serum control for the dose 0.1 cc. 
suspected serum 
3 1 | 0.2 1 1 1 1 | Test tube for the dose 0.2 cc. of 
of suspected serum 
4 De) OR | asrieveeeo 1 1 1 | Serum control for the dose 0.2 ce. 
suspected serum 
2 
5 eS besaileggetne 1 1 1 1 | Control for the quantity of ex- 
tract used (hemolysis) 
Ge | sssrgwisbone aaa 2 1 1 1 | Control for the double quantity of 
ext. for gr. accuracy (hemo.) 
7 ON Nei ck cdlletaaeetaiye 1 1 1 | Control of the hemolytic system 
(hemolysis) 
8 Bs Maencsniacal ieyeaos Fy | lheveeersss 1 | Control of the complement (no 
hemolysis) 
9 ON Seesect cs cea ee Says eeu 1 1 | Control of amboceptor (no hemo- 
lysis) 
10 A | ses as ob evn canst levies Semis orc 1 | Control of salt solution (no 
hemolysis) 
1. 0.9 per cent. NaC] solution. 
2. Suspected horse serum to be inactivated for 30 minutes at 56°-57° C. in water 
bath, in order to destroy the complement which is present in the serum of every horse. 
3. One-half of the quantity which does not prevent hemolysis and established by 
titration (See Table V). 
4. The determined smallest quantity established according to the preliminary test 
(See Table III). 
5. Double the quantity previously determined by titration (See Table IT). 
6. 5% suspension of washed sheep blood corpuscles. 
The necessary quantity has been established by Schtitz and Schubert as 0.2 and 0.1 
ce. placed in two different tubes. In some instances the tube containing 0.2 cc. may 
show a fixation of the complement, while the tube containing 0.1 cc. of the same serum 
may show only a partial fixation or hemolysis. In the majority of cases, however, the 
fixation is usually manifested in both tubes. 
Method of performing test. Four tubes are employed for testing the serum of each 
animal. Two tubes are used for the test proper and two for controls. The tubes are 
*Place for 1 hour in incubator or one-half hour in water bath at 37° C. 
