232 NECROBACILLOSIS 
ground-up tissue from a necrotic lesion. The tissues should be taken 
at the edge rather than from the center of the necrotic mass. When 
about 0.5 cc. of this is injected, death usually occurs within a week. 
The characteristic lesions will be found at the point of inoculation 
where lying beneath the skin and extending for a greater or less depth 
in the muscular tissue is an irregular area from 2 cm. to 5 cm. in 
diameter, consisting of a soft, putty-like appearance of necrosed tissue. 
It also has a penetrating odor resembling one between cheese and 
glue. In order to secure a pure culture of the necrosis bacillus, it is 
often necessary to inoculate a second rabbit and sometimes a third 
in a series. In later inoculations in which there are few organisms 
other than the one in question, much longer time is required for the 
death of the rabbit. In these cases, from eight to twenty or more days 
are required for the death of the rabbit and lesions may be found in the 
lungs, liver, kidneys and possibly other organs. From these second- 
ary areas of necrosis in the organs pure cultures may be obtained by 
the use of anzrobic methods. B. necrophorus is an obligatory 
anzerobe. 
REFERENCES 
1. Caupwetu. Case of equine bacillary necrosis, with metastatic lung lesions. 
Journ. Comp. Path. and Therap., Vol. XVII (1904), p. 65. 
2. Dammann. Die Diptherie der Kalber, eine neue, auf den Menschen tbertrag- - 
bare Zoonose. Deut. Zeitschr. f. Thiermed., Bd. III (1877), S. 1. 
3. Davis. Gangrenous dermatitis. Vet. Journ., Vol. XLIV (1897), p. 99. 
4. Davis. Gangrenous dermatitis. Vet. Journ., N.S., Vol. 1 (1900), p. 162. 
5. Eperizrm. Die Nekrose der Huflederhaut des Pferdes und ihre Behandlung. 
Monatsh. f. prakt. Thierheilk., Bd. VII (1896), S. 529. 
6. ExsertaH. Zwei Mykosen des Meerschweinchens. Virchow’s Arch., Vol. C 
(1885), p. 15. 
7. Ernst. Ueber Nekroses und den Nekrosebacillus (Streptothrix necrophora). 
Monatsheft f. prakt. Tierheilk., Bd. XIV. (1903), S. 193. 
8. Frouner. Dermatitis gangraenosa. In Bayer and Frohner’s Handbuch der 
tierdratlichen Chirurgie und Geburtshilfe, Bd. II, S. 284. 
9. Hess. Die Klauenkrankheiten des Rindes. Landwirthschaftl.jahrb. der Schweiz., 
Bd. VI (1893), S. 333. 
10. LignrErzs anp Spitz. Contribution a l’étude A la classification et a la 
nomenclature des affections connues sous le nom d’Actinomycose. Rec. d. méd. vét., 
Vol. LX XXII (1905), p. 64. 
11. Metvry anp Moutsr. Lip-and-Leg-ulceration of sheep. Cir. 160. B.A.1., 
1910. 
12, Monier anp Morse. Bacillus necrophorus and its economic importance. 
Ann. Report of B. A. I., 1904, p. 76. 
13. Moxurer anp Morsg. Necrotic stomatitis with special reference to its occur- 
rence in calves (calf diphtheria) and pigs (sore mouth). Bull. No. 67, B. A. I., 1904, 
p. 48. : 
